Journal: Frontiers in Immunology
Article Title: Predictive biomarkers and initial analysis of maternal immune alterations in postpartum preeclampsia reveal an immune-driven pathology
doi: 10.3389/fimmu.2024.1380629
Figure Lengend Snippet: Elevated blood pressures in patients with PPPE. Immunohistological analysis of the placenta revealed that patients who went on to develop PPPE had elevated macrophage marker (CD163) (A) along with increased Heme Oxygenase 2 (HO2) scores (B) . Representative images of immunohistochemistry staining for each marker are shown for CTL and PPPE patients (A, B) with black and white arrowheads indicating staining. Additional signs of prenatal initiation were evidenced by blood pressures taken at the 1 st trimester. Mean arterial blood pressures (MAP) were calculated using the systolic and diastolic pressures and revealed that patients with clinically uncomplicated pregnancies but who later developed PPPE had higher MAP (C) which was independently true for both systolic (D) and diastolic (E) blood pressures, while remaining below the hypertensive cut off of 140/90mmHg (dotted line) (C-E) . Bar graphs are presented as mean ± SEM. Box and whisker plots are presented with 10-90 percentile points. Statistics by unpaired t-tests where *p<0.05, **p<0.01, and ***p<0.001. Scale bar: 100μm.
Article Snippet: The following antibodies were used (dilution; concentration; catalog number, company): mouse anti-CD163 (1:100; 0.2mg/100μl; MCA1853, Bio-Rad), mouse anti-HO2 (1:50; 0.74mg/mL; NBP2-01407, Novus Biologicals), and rabbit anti-LC3b (1:100; 1mg/mL; NBP2-46892, Novus Biologicals).
Techniques: Marker, Immunohistochemistry, Staining, Whisker Assay